Fibrous Adsorbents as Novel Chromatography Matrices for Enhancing Industrial Downstream Processing of Bioproducts

  • The high demand for the production of biopharmaceuticals is limited by the total available manufacturing capacity, process efficiency and cost in a biotechnology industry mainly due to bottlenecks in the downstream processing (DSP) of the products. Downstream purification by traditional packed bed chromatography adsorbents plays a central role which exhibit major limitations, as a consequence the biopharmaceutical industry requires alternative materials and systems to replace. This PhD thesis is aimed at developing adsorption systems based on novel ion-exchange fibrous adsorbents for efficient and cost effective solutions to industrial downstream processing of bioproducts. In this work, strong cation exchange composite fibrous adsorbents have been developed and characterized. The mentioned system showed high capacity and high throughput for chromatography performance that can be used in intermediate purification and also exhibited superior performance during early protein capture application utilizing a model protein from an artificial crude feed stock. Similarly, strong anion-exchange fibrous adsorbents have been developed and characterized as high capacity chromatography support and investigated for their mass transfer properties. The fibrous geometry system demonstrated the potential for use in column scalable operations with high binding capacity and high throughput. A scaling prototype chromatography cartridge system based on the functionalized fibrous adsorbents has been evaluated and demonstrated superior hydrodynamics and process performances compared to commercial columns for industrial downstream processing. Finally, in this research work, the real application of fibrous base adsorbents has been successfully demonstrated as chromatographic support as an alternative to commercial beads in a single step capture and large-scale purification of an algal product, beta-Phycoerythrin, from unicellular marine algae using the mentioned system.

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Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Author:Poondi Rajash Gavara
Referee:Marcelo Fernandez Lahore, Georgi Muskhelishvili, Claudia Thomsen, Rami Reddy Vennapusa
Advisor:Marcelo Fernandez Lahore
Persistent Identifier (URN):urn:nbn:de:101:1-201305294977
Document Type:PhD Thesis
Language:English
Date of Successful Oral Defense:2012/06/04
Date of First Publication:2012/11/21
PhD Degree:Biochemical Engineering
School:SES School of Engineering and Science
Library of Congress Classification:T Technology / TP Chemical technology / TP248.13-248.65 Biotechnology / TP248.3 Biochemical engineering. Bioprocess engineering
Call No:Thesis 2012/38

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