TolC imaging and time-resolved production via fluorescent labeling in Escherichia coli

  • TolC is a component of the protein complex that forms the main bacterial efflux system involved in expelling out of the cell numerous harmful antibacterial chemicals such as antibiotics and detergents. TolC is the outer membrane component of the system and it connects with an inner membrane energy-demanding pump, which can be either an ABC transporter or a proton antiporter. A periplasmic membrane fusion protein seals the connection of the two. TolC is able to bind a multitude of such transporters which have very different substrate specificities thus TolC part-taking in the efflux of dozens of substrates. This makes TolC be a main participant in the phenomenon of multidrug efflux and a better understanding of its behavior in vivo is thus required. In the present study, the spatial localization and time-resolved dynamics of TolC production in live cells is described. This is achieved by labeling the TolC protein genomically with an oxidation-resistant variant of GFP, superfolder GFP. The labelled protein is fully functional and shows a growth medium-dependent expression. Furthermore, certain antibiotics increase its production in a concentration-dependent manner. This is the first time such a behavior was shown and opens new ways for further research.

The full text is embargoed until:

2017/11/11

Cite this publication

  • Export Bibtex
  • Export RIS

Citable URL (?):

Search for this publication

Search Google Scholar Search Catalog of German National Library Search OCLC WorldCat Search Catalog of GBV Common Library Network Search Catalog of Jacobs University Library Search Bielefeld Academic Search Engine
Meta data
Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Author:Alexander Mircea Petrescu
Referee:Mathias Winterhalter, Roland Benz, Malcolm Page, Jean-Michel Bolla
Advisor:Mathias Winterhalter
Persistent Identifier (URN):urn:nbn:de:gbv:579-opus-1007067
Document Type:PhD Thesis
Language:English
Date of Successful Oral Defense:2016/05/11
Date of First Publication:2017/05/11
Full Text Embargo Until:2017/11/11
Academic Department:Life Sciences & Chemistry
PhD Degree:Biochemical Engineering
Focus Area:Health
Call No:Thesis 2016/59

$Rev: 13581 $