Methods for characterization of bacterial channel using electrophysiology

  • This thesis emphasizes an interdisciplinary approach to comprehend the mechanisms of antibiotic translocation across the cell membrane. The single channel electrophysiology experiments were employed to obtain durable artificial lipid bilayer even at high voltages and molecular detail of antibiotic permeation through the outer membrane protein channels. The interactions of antibiotics with the outer membrane protein channels from Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa which have been studied investigation of three different subjects: the first study is carried out with a capillary-integrated compact microfluidic chip. The system is not only enabling the solvent-free bilayer experiments on a chip but also allows single protein detection and its antibiotic permeability through their channels with the low sample consumption. In the second study, we mainly investigate the interaction of phosphonic acid class of antibiotics with OprO and OprP porins from Pseudomonas aeruginosa at a single molecular level. In the third study, we demonstrate a possible stabilization approach for lipid bilayers using polydopamine deposition from dopamine containing solution. In addition to that, the study allows us to control translocation of antibiotic molecules through bacterial nanopores and use it as a biosensor for future applications.

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Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Referee:Mathias Winterhalter, Richard Wagner, Karsten Gall
Advisor:Mathias Winterhalter
Persistent Identifier (URN):urn:nbn:de:gbv:579-opus-1007305
Document Type:PhD Thesis
Date of Successful Oral Defense:2017/07/14
Date of First Publication:2017/08/04
Full Text Embargo Until:2018/08/03
Academic Department:Life Sciences & Chemistry
PhD Degree:Biochemical Engineering
Focus Area:Health
Call No:Thesis 2017/15

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