Molecular and ecological analysis of cellular attachment and induction of transparent exopolymeric particle formation in diatom-bacteria interactions

  • Transparent exopolymeric particles (TEP) and marine snow aggregates are vital components of the oceanic carbon cycle, leading to a substantial fraction of organic carbon sinking to depth or being provided for further recycling. Diatom-associated bacteria have been recently shown to directly impact TEP production and aggregate formation. However, very little is known about the molecular components that govern this interaction or the dynamics of TEP production and marine aggregate formation under changing environmental conditions. By combining molecular techniques and ecological experiments, we use an interdisciplinary approach to unveil hitherto unknown processes of diatom-bacteria interactions. As part of a collaborative and concerted effort, we initially established a genetically accessible bilateral model system consisting of the diatom Thalassiosira weissflogii and the marine gammaproteobacterium Marinobacter adhaerens HP15. Herein, we taxonomically established M. adhaerens HP15 as a novel member of the Marinobacter genus, revealed its genome sequence and established a genetic system to allow for the precise manipulation of this bacterium at the molecular level. In a second part, we used the established genetic toolbox to investigate the role of M. adhaerens HP15s motility during its interaction with the diatom. By generating M. adhaerens HP15 flagellum- and MSHA type IV pilus-deficient mutants, we demonstrate that a fullyfunctional flagellum is a pre-requisite for the bacterial attachment to both abiotic and diatom surfaces. We further show that the MSHA type-IV pilus is important for attachment, albeit to a lesser extent. Although both cellular appendages were shown to be crucial for attachment to diatom surfaces, this type of attachment was demonstrated to not be essential for inducing the formation of diatom-borne transparent exopolymeric particles (TEP). In the final part of this work, how TEP production dynamics and aggregate formation might be impacted in putative future oceanic scenarios was investigated. The results of our study suggest that the combined effect of ocean acidification and increased temperature might lead to a significant reduction in aggregate formation and sinking velocities of marine aggregates. We suggest that a combination of ocean acidification and global warming may severely impact the vertical transport of particulate organic matter in a future ocean.

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Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Author:Shalin Seebah
Referee:Matthias Ullrich, Laurenz Thomsen, Uta Passow
Advisor:Matthias Ullrich
Persistent Identifier (URN):urn:nbn:de:101:1-2013052812564
Document Type:PhD Thesis
Language:English
Date of Successful Oral Defense:2012/03/23
Date of First Publication:2012/04/27
PhD Degree:Marine Microbiology
School:SES School of Engineering and Science
Other Countries Involved:United States of America
Library of Congress Classification:G Geography. Anthropology. Recreation / GC Oceanography / GC109-149 Chemical oceanography
Call No:Thesis 2012/6

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