Influence of Lipopolysaccharides (LPS) on Antibiotic Permeation

  • The outer membrane of Gram-negative bacteria and the interaction of different antimicrobial agents were in the focus of this work. It could be shown that classical antibiotics like enrofloxacin as well as different antimicrobial peptides interact with membrane components such as lipopolysaccharides (LPS) as well as with membrane proteins. The outer membrane protein which was investigated in this study was the outer membrane protein F (OmpF). It showed different behaviours if it was reconstituted into membranes depending on the membrane composition and different interactions with various antimicrobial agents. The antimicrobial agents which were used in this work were enrofloxacin, as one example for classical antibiotics (quinolones), and a selection of different antimicrobial peptides including LL20, polymyxin B, hBD-3-l, and poly-Llysine. All of these substances were tested to determine if they showed interactions with LPS and OmpF utilizing several methods. The methods applied were biophysical as well as biological techniques like the Montal-Mueller technique, Foerster resonance energy transfer (FRET) spectroscopy, polarisation measurements, the analysis of the minimal inhibitory concentration, and several others. The results of this study indicate complex interactions of different membrane components of the Gram-negative bacterial outer membrane with different antimicrobial agents. All components and substances influence each other.

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Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Author:Annemarie Brauser
Referee:Mathias Winterhalter, Thomas Gutsmann, Jürgen Fritz
Advisor:Mathias Winterhalter
Persistent Identifier (URN):urn:nbn:de:101:1-2013052812494
Document Type:PhD Thesis
Language:English
Date of Successful Oral Defense:2011/05/24
Year of Completion:2011
Date of First Publication:2012/05/21
PhD Degree:Biochemical Engineering
School:SES School of Engineering and Science
Other Organisations Involved:Forschungszentrum Borstel Leibniz-Zentrum für Medizin und Biowissenschaften
Library of Congress Classification:R Medicine / RS Pharmacy and materia medica / RS153-441 Materia medica / RS400-431 Pharmaceutical chemistry
Call No:Thesis 2011/57

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